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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
Pageruler Plus Prestained Protein Ladder, supplied by Thermon Group Holdings, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
Pagerulertm Plus Prestained Protein Ladder Pi26620, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
Prestained Protein Ladder–Blue Plus® Iv (10–180 Kda), supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
Pageruler Plus Prestained Protein Ladder 10 250kd, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and <t>PageRuler™</t> Plus <t>Prestained</t> Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.
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INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and PageRuler™ Plus Prestained Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Expression of insulin-like 3 (INSL3) and differential splicing of its receptor in the ovary of rhesus macaques

doi: 10.1186/1477-7827-8-150

Figure Lengend Snippet: INSL3 protein expression in selected macaque tissues . a . Western blot analysis of INSL3 in selected endocrine-reproductive tissues. Eighty μg of total protein isolated from the ovary (Ov), testis (Te), hypothalamus (Hyp) and pituitary (Pit) was loaded in each lane. β-ACTIN (~47 kDa) was used as an internal loading control, and PageRuler™ Plus Prestained Protein Ladder (Fermentas) was used as molecular weight marker (MW). b . Immunohistochemistry (IHC) detection of INSL3 protein in the macaque ovary. Positive signal (brown) of INSL3 was localized in the thecal cells (The) surroundingantral follicles (AF), but not in other cell types within the ovary (A, B). Normal rabbit serum stained ovary section was used as negative control (C). GC, granulosa cells. c . INSL3 levels in monkey sera and follicular fluid during controlled ovarian stimulation (COS) protocols at 0-h and 36-h post hCG treatment. F serum follicular, female serum at follicular phase; F serum luteal, female serum at luteal phase; M, male. The value is presented as mean ± SE. Tissue lysate, sections, blood and follicular fluid were isolated from 3-4 different animals.

Article Snippet: The size of the detected protein band was determined by the PageRuler™ Plus Prestained Protein Ladder (Fermantas International Inc., Burlington, Ontario, Canada) separated on the same gel.

Techniques: Expressing, Western Blot, Isolation, Molecular Weight, Marker, Immunohistochemistry, Staining, Negative Control